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Key Documents

I2002

Sigma-Aldrich

异柠檬脱氢酶 (NADP) 来源于猪心脏

Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein

同義詞:

ICDH, IDH, 苏式-Ds-异柠檬酸: NADP+ 氧化还原酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

種類

Type IV

形狀

buffered aqueous glycerol solution

品質

≤ 0.2% of main activity is obtained with NAD as cofactor.

比活性

3-20 units/mg protein

成份

Protein biuret

運輸包裝

wet ice

儲存溫度

2-8°C

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應用

异柠檬酸脱氢酶(IDH)已用于:
  • 体外活性测定,用于研究钍对线粒体呼吸途径中柠檬酸循环酶的影响
  • 作为测量温度对烟酰胺二磷酸(NADP)-IDH 活性影响的标准品
  • 异柠檬酸盐含量测定

生化/生理作用

异柠檬酸脱氢酶(IDH)是一种同源二聚酶,参与适应缺氧、组蛋白去甲基化和 DNA 修饰等一些过程。这种酶有三种异构体,如 IDH1、IDH2 和 IDH3

單位定義

在37℃条件下,当pH为7.4时,一个单位每分钟将1.0 μ摩尔的异柠檬酸盐转化为至α-酮戊二酸。

外觀

50%甘油EDTA缓冲盐溶液,pH 6.0

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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Erdem M Terzi et al.
Science advances, 7(22) (2021-05-28)
Intracellular iron levels are strictly regulated to support homeostasis and avoid iron-mediated ROS production. Loss of iron-sulfur cluster (ISC) synthesis can increase iron loading and promote cell death by ferroptosis. Iron-responsive element-binding proteins IRP1 and IRP2 posttranscriptionally regulate iron homeostasis.
B C Medeiros et al.
Leukemia, 31(2), 272-281 (2016-10-11)
Alterations to genes involved in cellular metabolism and epigenetic regulation are implicated in the pathogenesis of myeloid malignancies. Recurring mutations in isocitrate dehydrogenase (IDH) genes are detected in approximately 20% of adult patients with acute myeloid leukemia (AML) and 5%
Avtar Singh et al.
The Journal of biological chemistry, 278(35), 33208-33216 (2003-06-07)
Transhydrogenase couples the reduction of NADP+ by NADH to inward proton translocation across mitochondrial and bacterial membranes. The coupling reactions occur within the protein by long distance conformational changes. In intact transhydrogenase and in complexes formed from the isolated, nucleotide-binding
M Ryan Smith et al.
Redox biology, 8, 136-148 (2016-01-18)
Many cancer cells follow an aberrant metabolic program to maintain energy for rapid cell proliferation. Metabolic reprogramming often involves the upregulation of glutaminolysis to generate reducing equivalents for the electron transport chain and amino acids for protein synthesis. Critical enzymes
Icksoo Lee et al.
Biochimica et biophysica acta, 1802(2), 275-283 (2009-10-20)
Noonan syndrome (NS) is an autosomal dominant disorder, and a main feature is congenital heart malformation. About 50% of cases are caused by gain-of-function mutations in the tyrosine phosphatase SHP2/PTPN11, a downstream regulator of ERK/MAPK. Recently it was reported that

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