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重要文件

P4032

Sigma-Aldrich

朊酶 来源于蜂蜜曲霉

Type XXIII, ≥3 units/mg solid

同義詞:

Protease from Aspergillus melleus, Proteinase from Aspergillus sp.

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物源

Aspergillus sp. (A. melleus)

品質等級

種類

Type XXIII

形狀

solid

比活性

≥3 units/mg solid

儲存溫度

2-8°C

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應用

蛋白酶是一种通过水解肽键来分解蛋白质的酶。蛋白酶用于降解蛋白质、研究蛋白酶抑制剂和研究热灭活动力学。蛋白酶用于孵育过程中的核酸分离程序。它被用于研究蛋白酶激活受体,如炎症和免疫反应中蛋白酶介导的信号转导 。产品 P4032 来自 黑曲霉 ,已用于霍氏链霉菌木聚糖酶的非特异性降解。

生化/生理作用

蛋白酶通过水解肽键使蛋白质分解代谢。丝氨酸活性部位抑制剂可灭活蛋白酶,如苯甲基磺酰氟 (PMSF) 和二异丙基氟磷酸盐。

單位定義

除非另有说明,否则一个单位将水解酪蛋白以在 pH7.5,37°C 下每分钟产生相当于1.0μmole (181μg) 酪氨酸的颜色(Folin-Ciocalteu 试剂的颜色)。

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Endocrine reviews, 26(1), 1-43 (2005-02-04)
Serine proteinases such as thrombin, mast cell tryptase, trypsin, or cathepsin G, for example, are highly active mediators with diverse biological activities. So far, proteinases have been considered to act primarily as degradative enzymes in the extracellular space. However, their
J C Groot et al.
The British journal of nutrition, 79(6), 519-525 (1998-10-15)
Differences between the fermentation characteristics of cell contents (CC) and protease-treated cell walls (CW) of young leaves of Italian ryegrass (Lolium multiflorum Lam.) cultivar Multimo (tetraploid), were studied in vitro. Gas and volatile fatty acid (VFA) production rates were measured
José M Fernández-Abalos et al.
Microbiology (Reading, England), 149(Pt 7), 1623-1632 (2003-07-12)
The xylanase Xys1L from Streptomyces halstedii JM8 is known to be processed extracellularly, to produce a protein of 33.7 kDa, Xys1S, that retains catalytic activity but not its cellulose-binding capacity. This paper demonstrates that at least five serine proteases isolated
Mikhail E Kandel et al.
Nature communications, 10(1), 4691-4691 (2019-10-18)
Multiple scattering and absorption limit the depth at which biological tissues can be imaged with light. In thick unlabeled specimens, multiple scattering randomizes the phase of the field and absorption attenuates light that travels long optical paths. These obstacles limit

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To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.

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