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Key Documents

P3303

Sigma-Aldrich

内蛋白酶Asp-N 来源于莓实假单胞菌 突变菌株

suitable for protein sequencing, lyophilized powder

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.56

等級

Proteomics Grade

品質等級

形狀

lyophilized powder

分析物化學類別

amino acids

包裝

vial of 2 μg

適合性

suitable for protein sequencing

儲存溫度

2-8°C

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一般說明

胞内蛋白酶 Asp-N 是一种金属内切蛋白酶。它来自于 莓实假单胞菌 的一个突变菌株,该菌株水解天冬氨酸和半胱氨酸残基 N 端的肽键。 Asp-N 因其对多肽的高度特异性剪切,在蛋白质组学中用于肽图分析和蛋白质序列工作。

應用

来自莓实假单胞菌突变菌株的胞内蛋白酶 Asp-N 已用于特定蛋白质的消化,以制备肽并通过 MS(质谱)法分析生成的肽。

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


分析證明 (COA)

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T H Jensen et al.
The Journal of biological chemistry, 270(23), 13777-13784 (1995-06-09)
Human immunodeficiency virus encodes the regulatory protein Rev, which is required for expression of viral structural proteins. It binds to an RNA element (RRE) in the viral transcript and up-regulates the cytoplasmic appearance of unspliced and singly spliced viral mRNA.
Wilson KJ, et al.
Methods in Protein Sequence Analysis: Proceedings of the 7th International Conference, Berlin, July 3?8, 1988, 310-310 (1988)
Yeast ribosomal/cytochrome c SET domain methyltransferase subfamily: identification of Rpl23ab methylation sites and recognition motifs.
Kameoka D, et al.
Journal of Biochemistry, 134, 129-135 (2003)
S R Paik et al.
The Biochemical journal, 340 ( Pt 3), 821-828 (1999-06-09)
alpha-Synuclein is a component of the abnormal protein depositions in senile plaques and Lewy bodies of Alzheimer's disease (AD) and Parkinson's disease respectively. The protein was suggested to provide a possible nucleation centre for plaque formation in AD via selective
Sarah Murray et al.
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