推薦產品
形狀
lyophilized (salt-free)
比活性
20 U/mg
分子量
30 kDa
包裝
pkg of 2 mg
製造商/商標名
Roche
最適pH
8.0-8.5
一般說明
内切蛋白酶Glu-C是一种葡萄球菌丝氨酸蛋白酶。其抑制剂包括DFP、α2-巨球蛋白、以及TLCK。
Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).
At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).
特異性
热灭活:通过煮沸10分钟使内切蛋白酶Glu-C失活。
應用
使用内切蛋白酶Glu-C(V8蛋白酶)进行蛋白结构分析和序列分析。
生化/生理作用
内切蛋白酶Glu-C可特异性地水解Glu(或同时水解Glu和Asp)羧基侧的肽和酯键,其取决于所用的缓冲液。
準備報告
激活剂:该酶在SH试剂存在下具有最大活性
工作浓度:1 至 5 mM
工作溶液: 推荐的溶剂是50mM乙酸铵(pH 4.0,2mg/ml)。
储存条件(工作溶液):-15至-25°C
分液冷冻保存并仅解冻一次,酶(2mg/ml,溶于50mM乙酸铵中,pH 4.0)可稳定保存至少一个月。
工作浓度:1 至 5 mM
工作溶液: 推荐的溶剂是50mM乙酸铵(pH 4.0,2mg/ml)。
储存条件(工作溶液):-15至-25°C
分液冷冻保存并仅解冻一次,酶(2mg/ml,溶于50mM乙酸铵中,pH 4.0)可稳定保存至少一个月。
儲存和穩定性
在2°C至8°条件下储存。 (请保持干燥!)
其他說明
仅用于生命科学研究。不可用于诊断。
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
does not flash
閃點(°C)
does not flash
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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