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Key Documents

C100

Sigma-Aldrich

过氧化氢酶 来源于牛肝脏

aqueous suspension, 40,000-60,000 units/mg protein (E1%/405)

同義詞:

H2O2:H2O2 氧化还原酶

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

形狀

aqueous suspension

品質等級

比活性

40,000-60,000 units/mg protein (E1%/405)

分子量

tetramer ~250 kDa

等電點

5.4

UniProt登錄號

運輸包裝

wet ice

儲存溫度

2-8°C

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

InChI 密鑰

NUVBSKCKDOMJSU-UHFFFAOYSA-N

基因資訊

cow ... CAT(280743)

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一般說明

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

應用

过氧化氢酶作为天然抗氧化剂,可用于研究活性氧物质对基因表达和凋亡的作用。它还被用于保护蛋白质、脂质和核酸免受氧化损伤。 工业上,过氧化氢酶已被用于去除添加至牛奶和奶酪中以及纺织品漂白使用的过氧化氢,并用于检查其对DNA修复大肠杆菌突变体活力的阳性效应。

生化/生理作用

Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.

注意

过氧化氢酶溶液不可冷冻。 冷冻溶液将导致酶活性损失50-70%。

單位定義

一单位酶可在pH7.0、25℃条件下每分钟分解1.0 μM的H2O2,通过测量A240的下降速率可知H2O2的浓度从10.3降至9.2 mM。

準備報告

本品为含0.1%(w/v)百里香酚的过氧化氢酶水悬浮液,活性为40,000-60,000单位/mg蛋白质。为除去百里香酚防腐剂,可以通过离心使过氧化氢酶晶体沉淀,弃去上清液,将沉淀物重悬于水中,然后再次沉淀。该酶溶于50 mM磷酸钾缓冲液中,浓度为1 mg/mL,pH 7.0。

抑制劑

產品號碼
描述
訂價

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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