推薦產品
種類
Type XVII-B
品質等級
形狀
lyophilized powder
比活性
500-1,000 units/mg solid
分子量
29 kDa
純化經由
chromatography
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
Staphylococcus aureus subsp. aureus MW2 ... sspA(1003044)
尋找類似的產品？ 前往 產品比較指南
應用
内切蛋白酶Glu-C（来自金黄色葡萄球菌 Staphylococcus aureus V8）已用于消化还原和烷基化环肽以生产线性片段。
它可用于蛋白质选择性切割以便进行氨基酸序列测定或肽谱分析。
来自 Staphylococcus aureus菌株V8的内切蛋白酶Glu-C是一种丝氨酸蛋白酶，可用于蛋白质选择性切割，用来进行氨基酸序列确定或多肽图谱。 产品P2922用于线性化蝶豆叶子提取物中的环状多肽。
生化／生理作用
在磷酸缓冲液中，Staphylococcus菌株V9蛋白酶特异性地切割多肽链天冬氨酸和谷氨酸残基的羧基侧。 当在碳酸氢铵缓冲液或醋酸铵中时，只切割谷氨酸残基的羧基侧。 该酶在pH4.0到7.8之间有最大活性。如果使用血红蛋白为底物，最大活性在pH4.0。 当酪蛋白为底物，最大活性在pH7.8。
單位定義
1单位酶可在pH7.8,37℃条件，每分钟水解1 μmole N-t-Boc--L-谷氨酸α-苯酯。一单位相当于 ~0.004单位酪蛋白消化所需的酶量。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
客戶也查看了
From the Cover: Discovery of an unusual biosynthetic origin for circular proteins in legumes
Aaron G. Poth, Michelle L. Colgrave, et al.
Proceedings of the National Academy of Sciences of the USA, 108 (2011)
Shanshan Liu et al.
Amino acids, 48(4), 1059-1067 (2016-01-11)
Common yet often overlooked, deamidation of peptidyl asparagine (Asn or N) generates aspartic acid (Asp or D) or isoaspartic acid (isoAsp or isoD). Being a spontaneous, non-enzymatic protein post-translational modification, deamidation artifact can be easily introduced during sample preparation, especially
Andrew Michael Frey et al.
Cell reports, 35(1), 108930-108930 (2021-04-08)
Staphylococcus aureus possesses ten extracellular proteases with mostly unknown targets in the human proteome. To assist with bacterial protease target discovery, we have applied and compared two N-terminomics methods to investigate cleavage of human serum proteins by S. aureus V8 protease
Feliciano Priego-Capote et al.
Methods in molecular biology (Clifton, N.J.), 728, 219-232 (2011-04-07)
Glucose is the predominant source of energy in cells. However, a chronic high glucose exposure of proteins modifies a number of biological pathways, known as glucotoxicity. Several studies have suggested that this impaired protein function is associated in part to
Chengjian Tu et al.
Analytical chemistry, 83(12), 4802-4813 (2011-04-16)
The plasma proteome holds enormous clinical potential, yet an in-depth analysis of the plasma proteome remains a daunting challenge due to its high complexity and the extremely wide dynamic range in protein concentrations. Furthermore, existing antibody-based approaches for depleting high-abundance
條款
This procedure may be used for determination of Endoproteinase Glu-C enzymatic activity using N-t-Boc-L-glutamic acid α-phenyl ester as the substrate.
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務