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NA0300S

Sigma-Aldrich

GenElute HP质粒大量制备试剂盒

greener alternative

sufficient for 4 purifications

同義詞:

GenElute HP质粒大量制备试剂盒

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.52

用途

sufficient for 4 purifications

環保替代產品特色

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

DNA purification: suitable

環保替代類別

儲存溫度

15-25°C

一般說明

GenElute HP质粒大量制备试剂盒可以简单、快速、低成本的从重组大肠杆菌培养物中分离质粒DNA。该试剂盒采用了一种可快速清除裂解液的过滤针筒和一种适于真空或离心形式的硅胶结合柱。从150 mL Luria Broth (LB)培养基过夜培养物中,可分离出多达1.2 mg 的质粒DNA。请注意,实际得率取决于所用的菌株、质粒和培养基。

通过离心分离法收集过夜培养的重组大肠杆菌培养物,并通过改性SDS碱裂解法进行处理,之后再在高盐环境下使DNA吸附到硅胶膜上。通过两遍洗涤步骤,去除污染物。最后,在洗脱溶液(Tris-HCl)或水中洗脱结合所得的DNA。

回收的质粒DNA主要以超螺旋形式存在。此类DNA可直接供限制性酶切、连接、测序、PCR、转化和转染等下游应用使用。
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 (The 12 Principles of Greener Chemistry),更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法,本产品符合固有安全化学原则(Inherently Safer Chemistry)。

應用

GenElute HP Plasmid Maxiprep Kit has been used to isolate plasmid DNA from Escherichia coli.

特點和優勢

  • 可在30分钟内从收集所得的细菌培养物中纯化出质粒DNA
  • 可纯化出多达1.2 mg的高拷贝数质粒DNA
  • 真空或离心形式可灵活选择
  • 相对于其他快速试剂盒,所含的塑料成分更少,减少了废物量
  • 无需进行苯酚/氯仿萃取或乙醇沉淀步骤

其他說明

更多信息,请见www.sigma-aldrich.com/genelutehp

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

標靶器官

Central nervous system

儲存類別代碼

3 - Flammable liquids

閃點(°F)

77.0 °F - closed cup

閃點(°C)

25 °C - closed cup


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Lisa A Santry et al.
Molecular therapy. Methods & clinical development, 9, 181-191 (2018-03-21)
Newcastle disease virus (NDV) is a single-stranded, negative-sense RNA virus in the Paramyxoviridae family. Although primarily an avian pathogen, NDV is a potent oncolytic virus that has been shown to be safe and effective in a variety of preclinical cancer
Development of novel plasmid vectors and a promoter trap system in Francisella tularensis compatible with the pFLN10 based plasmids
David A Rasko
Plasmid, 58(2) (2007)
Ilaria Mogno et al.
Genome research, 23(11), 1908-1915 (2013-08-08)
Gene promoters typically contain multiple transcription factor binding sites (TFBSs), which may vary in affinity for their cognate transcription factors (TFs). One major challenge in studying cis-regulation is to understand how TFBS variants affect gene expression. We studied the in

條款

The GenElute™ HP Plasmid Maxiprep kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cutures. The recovered plasmid DNA is ready for immediate use in downstream applications, such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.

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