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Key Documents

N5386

Sigma-Aldrich

微球菌核酸酶 来源于金黄色葡萄球菌

100-300 units/mg protein

同義詞:

MNase, 微球菌核酸内切酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Staphylococcus aureus

品質等級

形狀

powder

比活性

100-300 units/mg protein

成份

Protein, ≥40% E1%/280 (Balance primarily sodium acetate)

技術

DNA extraction: suitable
DNA purification: suitable

適合性

suitable for molecular biology

應用

cell analysis

儲存溫度

−20°C

基因資訊

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

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應用

微球菌核酸酶可用于消化染色质以鉴定核小体的位置。
来源于金黄色葡萄球菌的微球菌核酸酶已被用于研究中监测与 DNA 的杂交反应。它也被用于研究葡萄球菌核酸酶测定的最佳条件。

生化/生理作用

可水解DNA的5′-磷酸二酯键。

其他說明

注意:1μmole单位 = 约85 A260 单位,其中A260单位相当于在pH 8.8,37℃条件下,在3.55 mL反应体积中,30分钟内ΔA260为1.0。光路= 1cm。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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存取文件庫

Optimal conditions for assay of staphylococcal nuclease
KAMMAN, J. and S. TATINI
Journal of Food Science, 42, 421-424 (1977)
Ho-Ryun Chung et al.
PloS one, 5(12), e15754-e15754 (2011-01-06)
Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the
Use of micrococcal nuclease to monitor hybridization reactions with DNA.
D L Kacian et al.
Analytical biochemistry, 58(2), 534-540 (1974-04-01)
Ashish Kumar Singh et al.
Nature communications, 12(1), 7011-7011 (2021-12-03)
Numerous chromatin remodeling enzymes position nucleosomes in eukaryotic cells. Aside from these factors, transcription, DNA sequence, and statistical positioning of nucleosomes also shape the nucleosome landscape. The precise contributions of these processes remain unclear due to their functional redundancy in
Amnat Phetrungnapha et al.
Fish & shellfish immunology, 34(3), 875-884 (2013-01-22)
RNA interference (RNAi) plays a crucial role as an antiviral defense in several organisms including plants and invertebrates. An understanding of RNAi machineries especially protein components of the RNA-induced silencing complex (RISC) is essential for prior to applying RNAi as

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