跳轉至內容
Merck
  • The effect of micrococcal nuclease digestion on nucleosome positioning data.

The effect of micrococcal nuclease digestion on nucleosome positioning data.

PloS one (2011-01-06)
Ho-Ryun Chung, Ilona Dunkel, Franziska Heise, Christian Linke, Sylvia Krobitsch, Ann E Ehrenhofer-Murray, Silke R Sperling, Martin Vingron
摘要

Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the cutting preference of MNase in combination with size selection generates a sequence-dependent bias in the resulting fragments. This strongly affects nucleosome positioning data and especially sequence-dependent models for nucleosome positioning. As a consequence we see a need to re-evaluate whether the DNA sequence is a major determinant of nucleosome positioning in vivo. More generally, our results show that data generated after MNase digestion of chromatin requires a matched control experiment in order to determine nucleosome positions.

材料
產品編號
品牌
產品描述

Sigma-Aldrich
微球菌核酸酶 来源于金黄色葡萄球菌, 100-300 units/mg protein