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Merck
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E1014

Millipore

Benzonase®核酸酶

≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

同義詞:

内切核酸酶 来源于粘质沙雷氏菌

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About This Item

CAS號碼:
9025-65-4
酶委員會編號:
3.1.30.2 (BRENDA, IUBMB)
MDL號碼:
MFCD00131010
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Serratia marcescens

品質等級

200

重組細胞

expressed in E. coli

化驗

≥90% (SDS-PAGE)

形狀

buffered aqueous glycerol solution

分子量

30 kDa

濃度

≥250 units/μL

應用

research use

異物活動

protease, essentially free

運輸包裝

wet ice

儲存溫度

−20°C

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相關類別

一般說明

Benzonase®全能核酸酶是一种通过高效基因工程法从粘质沙雷氏菌中获得的核酸内切酶。这种具有两个必需二硫键可在宽泛的操作条件下攻击并降解所有形式的DNA和RNA（单链、双链、线性和环化）。它能将核酸完全消化成长度为3到5个碱基的5′-单磷酸末端寡核苷酸，使其成为从重组蛋白中去除核酸的理想工具，并用于需要完全消化核酸的应用。除了降低蛋白质提取物的粘度和并防止细胞结团之外，使用Benzonase®全能核酸酶预处理蛋白质样本可以消除任何结合的核酸，进而显著提高2D凝胶电泳的分辨率。这种多功能酶可消化天然或热变性的DNA和RNA，其酶活性的最佳pH值为8.0-9.2。Benzonase®全能核酸酶能够去除核酸并提高蛋白质样本的纯度和质量。

應用

Benzonase® Nuclease has been used: as a component in ice-cold lysis buffer C to digest DNA and  RNA to facilitate the complete release of all nuclear proteins in the immunoprecipitation step to release protein complexes from the nucleoplasm and chromatinas a supplement in RIPA to fractionate SHSY5Y cells for immunoprecipitation to remove residual nucleic acids from the aortic roots in decellularization method
用于去除蛋白样品中的核酸。

生化／生理作用

Benzonase® Nuclease can completely digest nucleic acids into 5′-monophosphate terminated oligonucleotides of 3 to 5 bases in length, making it the ideal tool for removing nucleic acids from recombinant proteins and for applications that require complete digestion of nucleic acids. In addition to reducing viscosity in protein extracts and preventing cell clumping, pretreatment of protein samples with Benzonase® nuclease can significantly improve their resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. This versatile enzyme can digest both native or heat-denatured DNA and RNA, with its optimum pH for enzyme activity found to be 8.0-9.2. Benzonase® nuclease is effective at removing host DNA from microbiome samples. In many cases, microbiome samples (such as saliva or skin) will have a high percentage of host DNA that interferes with downstream results. Our experts show that the reduction of host DNA lowers the cost of sequencing while increasing and improving the data. Experimental data is shown in the technical article - Benzonase® Nuclease for Microbiome Workflows
消化天然或热变性的DNA和RNA。

特點和優勢

微生物组样本中宿主DNA的损耗

多种工作流程中核酸的有效消化

蛋白质提取过程中粘度的降低

單位定義

在pH 8.0，37 °C条件下，一个单位的酶在30分钟内可将超声的鲑鱼精子DNA消化成等同于1.0 ΔA260的酸可溶寡核苷酸（2.625 ml反应体积）。

外觀

在含有20 mM Tris HCl, pH 8.0, 2 mM MgCl2及20 mM NaCl的50%甘油中的溶液。

其他說明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

法律資訊

Benzonase®核酸酶由德国达姆施塔特默克公司和/或其附属公司提供。
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

分析證明 (COA)

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Jos J M Drabbels et al.
Blood, 118(19), e149-e155 (2011-09-21)
Microchimerism is defined by the presence of low levels of nonhost cells in a person. We developed a reliable method for separating viable microchimeric cells from the host environment. For flow cytometric cell sorting, HLA antigens were targeted with human
Janus S Jakobsen et al.
Science advances, 5(7), eaaw4304-eaaw4304 (2019-07-17)
The key myeloid transcription factor (TF), CEBPA, is frequently mutated in acute myeloid leukemia (AML), but the direct molecular effects of this leukemic driver mutation remain elusive. To investigate CEBPA mutant AML, we performed microscale, in vivo chromatin immunoprecipitation sequencing
T K Ball et al.
Gene, 57(2-3), 183-192 (1987-01-01)
We are studying exoproteins of the enteric bacterium Serratia marcescens as a model system for the release of extracellular proteins from the cell. In this work we report the cloning of the gene for a secreted nuclease from S. marcescens
T K Ball et al.
Nucleic acids research, 20(19), 4971-4974 (1992-10-11)
The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a
An extracellular nuclease from Serratia marcescens. II. Specificity of the enzyme.
M Nestle et al.
The Journal of biological chemistry, 244(19), 5219-5225 (1969-10-10)
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文章

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

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