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Key Documents

MS0100

Supelco

ProteoMass Guanidination Kit

For improving MALDI-MS sensitivity and peptide sequence coverage

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.24

品質等級

產品線

ProteoMass

應用

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

相容性

for use with (Complex cell extracts, pure protein solution, 1D and 2D PAGE gels)

應用

ProteoMass Guanidination Kit 有助于增强MALDI-MS 的灵敏性,提高序列覆盖度,更可靠地对多肽进行鉴定。蛋白水解消化后,C端带有精氨酸残基的多肽相比C端带赖氨酸残基的多肽会更优先离子化,从而使得多肽质谱分析中序列覆盖度受影响,可靠度有限。ProteoMass Guanidination Kit 可以高效方便地将C端赖氨酸残基转化为高精氨酸,提高MALDI的信号强度,并获得更好的序列覆盖度。

特點和優勢

  • 更精确可靠地鉴定更多的样品
  • 提高通量,节省时间—使用试剂盒仅需35分钟 vs. 传统方法需要2小时
  • 兼容性—可用于1D或2D的PAGE凝胶条带或点,也可用于复杂的细胞提取物。

其他說明

一盒MS0100 试剂盒可进行96次反应。

法律資訊

ProteoMass is a trademark of Sigma-Aldrich Co. LLC

相關產品

產品號碼
描述
訂價

訊號詞

Danger

危險聲明

危險分類

Aquatic Acute 1 - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Yoshimi Kanie et al.
PloS one, 4(5), e5434-e5434 (2009-05-06)
A variety of N-glycans attached to protein are known to involve in many important biological functions. Endoplasmic reticulum (ER) and Golgi localized enzymes are responsible to this template-independent glycan synthesis resulting glycoforms at each asparagine residues. The regulation mechanism such
Fumihiro Oshita et al.
Oncology reports, 24(3), 637-645 (2010-07-29)
To investigate the early changes in protein function that induce micro-metastasis in early-stage non-small cell lung cancer, we conducted proteomic analysis of tissue that had been completely resected. We selected sixteen patients whose tumors were pathological stage I adenocarcinoma with
Roberta Cozzi et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(5), 2008-2018 (2012-01-19)
Group B Streptococcus pili are covalently linked structures assembled via a sortase-catalyzed transpeptidation mechanism involving specific residues and motifs. A sequence element containing a conserved glutamic acid, called the E-box, has been described to be involved in pilus formation. Although
Alberto Nuñez et al.
Journal of agricultural and food chemistry, 57(22), 10951-10958 (2009-10-29)
Several studies have suggested that the emulsification properties associated with pectin obtained from sugar beet (Beta vulgaris) are due to the presence of a protein-pectin complex. Nevertheless, the identity of the protein has remained elusive. Pectin, extracted from sugar beet
Joungil Choi et al.
The Journal of biological chemistry, 281(16), 10816-10824 (2006-03-07)
Mutations in DJ-1 cause an autosomal recessive, early onset familial form of Parkinson disease (PD). However, little is presently known about the role of DJ-1 in the more common sporadic form of PD and in other age-related neurodegenerative diseases, such

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