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Merck
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重要文件

M7278

Sigma-Aldrich

Eagle最低必需培养基

HEPES modification, with Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

同義詞:

MEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

產品名稱

Eagle最低必需培养基, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

pH值

>7.2

成分

L-glutamine: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

EMEM培养基由糖、无机盐、水溶性维生素和必需氨基酸组成,广泛用于许多细胞培养基研究的培养基配方。

應用

EMEM培养基用于培养原始卵泡和卵巢组织、人体皮肤成纤维细胞、人结肠上皮细胞系 LS174T 和海马组织切片等。

重構

添加 0.292g/L L-谷氨酰胺。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

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RNA sequencing studies have identified hundreds of non-coding RNAs in bacteria, including regulatory small RNA (sRNA). However, our understanding of sRNA function has lagged behind their identification due to a lack of tools for the high-throughput analysis of RNA-RNA interactions
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Genetically encoded nanosensors such as the FRET-based adenosine triphosphate (ATP) sensor ATeam enable the measurement of changes in ATP levels inside cells, promoting our understanding of metabolic interactions between astrocytes and neurons. The sensors are usually well characterized in vitro
Alfred K Yu et al.
Human molecular genetics, 24(10), 2848-2860 (2015-02-06)
Mitochondrial complex I (NADH dehydrogenase) is a major contributor to neuronal energetics, and mutations in complex I lead to vision loss. Functional, neuroanatomical and transcriptional consequences of complex I deficiency were investigated in retinas of the Ndufs4 knockout mouse. Whole-eye
Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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