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Eagle最低必需培养基, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
無菌
sterile-filtered
形狀
liquid
技術
cell culture | mammalian: suitable
雜質
endotoxin, tested
成分
phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
Eagle最低必需培养基(MEM)是Harry Eagle开发的人工合成细胞培养基。培养基含有较高浓度的氨基酸,因此比较接近哺乳动物培养细胞的蛋白质成分。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸,可扩大该培养基的用途。通过选择性去除钙成分后,该配方可进一步改良为适合悬浮细胞培养。MEM可用于各种细胞的单层培养。
應用
Eagle最低必需培养基已用于培养:
此培养基在血清减量条件下适合贴壁细胞生长,也可作为无血清培养基开发的基础配方。
- HeLa细胞
- Madin-Darby犬肾细胞 (MDCK)
- 软骨肉瘤细胞系
此培养基在血清减量条件下适合贴壁细胞生长,也可作为无血清培养基开发的基础配方。
重構
添加 0.292g/L L-谷氨酰胺。
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產品號碼
描述
訂價
相關產品
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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