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Key Documents

M4655

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

同義詞:

MEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no
NaHCO3: yes
L-glutamine: yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

由Harry Eagle开发的最低必需培养基(MEM)是所有合成细胞培养基中使用最广泛的培养基之一。早期在尝试培养正常的哺乳动物成纤维细胞和HeLa细胞的某些亚型的过程中,显示它们具有Eagle's基础培养基(BME)无法满足的特定营养需求。随后在培养中使用这些细胞和其他细胞的研究表明,通过向BME中加入添加物以帮助更广泛的苛刻细胞的生长。

通过改良的MEM包括更高浓度的氨基酸,因此该培养基更接近于所培养的哺乳动物细胞的蛋白质组成。MEM已用于在单层中生长的各种细胞的培养。在含有Hanks'或Earle′s盐的配方中选择补充非必需氨基酸,可扩大该培养基的用途。

應用

通常用于在FBS、小牛或马血清存在下生长附着的细胞系,例如成纤维细胞。

其他說明

该产品中缺乏L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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