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M4526

Sigma-Aldrich

Eagle最低必需培养基

Alpha Modification, with sodium bicarbonate and Earl's salts, without ʟ-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

同義詞:

αMEM, MEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

產品名稱

Eagle最低必需培养基, Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

品質等級

400

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

運輸包裝

ambient

儲存溫度

2-8°C

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相關類別

一般說明

最小必需培养基Eagle（MEM）是一种合成细胞培养基，由Harry Eagle开发。它有更高浓度的氨基酸，所以培养基更接近培养哺乳动物细胞的蛋白质组成。在含有Hanks或Earle盐的配方中选择补充非必需氨基酸，可增强该培养基的用途。Earle平衡盐的MEM修饰也称为αMEM，含有非必需氨基酸、丙酮酸钠和其他维生素。
这是所提供的MEM配方中最丰富的变体。它含有所有21种正常氨基酸，其中一些的浓度有所增加。此外，它还含有5种额外的维生素。

應用

最小必需培养基Eagle已用于维持：
  • 小鼠未成熟的卵母细胞络合物
  • 人类间充质干细胞（MSC）
  • 小鼠成骨前细胞系

重構

添加 0.292g/L L-谷氨酰胺。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
RNBM0562
RNBL7706
RNBL2659
RNBL5431
RNBK8783

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Michelle L Churchman et al.
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Somatic genetic alterations of IKZF1, which encodes the lymphoid transcription factor IKAROS, are common in high-risk B-progenitor acute lymphoblastic leukemia (ALL) and are associated with poor prognosis. Such alterations result in the acquisition of stem cell-like features, overexpression of adhesion
Thi Thu Hien Bui et al.
Molecular reproduction and development, 84(5), 356-362 (2017-02-25)
Full-grown mouse antral oocytes are classified as surrounding nucleolus (SN) or not-surrounding nucleolus (NSN), depending on the respective presence or absence of a ring of Hoechst-positive chromatin surrounding the nucleolus. In culture, both types of oocytes resume meiosis and reach
Masaaki Kitada et al.
Glia, 67(5), 950-966 (2019-01-15)
Direct conversion is considered a promising approach to obtain tissue-specific cells for cell therapies; however, this strategy depends on exogenous gene expression that may cause undesired adverse effects such as tumorigenesis. By optimizing the Schwann cell induction system, which was
Ricardo Rodrigues-Pinto et al.
Scientific reports, 8(1), 12866-12866 (2018-08-29)
The adult nucleus pulposus originates from the embryonic notochord, but loss of notochordal cells with skeletal maturity in humans is thought to contribute to the onset of intervertebral disc degeneration. Thus, defining the phenotype of human embryonic/fetal notochordal cells is
Sarah Hemming et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(3), 1011-1027 (2016-12-10)
In this study, we examined the functional importance of EZH2 during skeletal development and homeostasis using the conditional deletion of
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