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M0644

Sigma-Aldrich

Eagle最低必需培养基

Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture

同義詞:

MEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

成分

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

最低必需培养基(MEM)是Harry Eagle开发的合成细胞培养基。培养基含有较高浓度的氨基酸,因此比较接近哺乳动物培养细胞的蛋白质成分。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸,可扩大该培养基的用途。混合Earle′s平衡盐的MEM是改良配方,又名αMEM,包含非必需氨基酸、丙酮酸钠和额外的维生素。

應用

Eagle最低必需培养基已用于:

  • 自然杀伤细胞(NK)的维持培养
  • 杜氏利什曼原虫(Leishmania donovani)菌株培养
  • 大鼠毛囊隆突部位表皮神经嵴干细胞(EPI-NCSCs)的培养

數量

按照每升培养基10.1克粉末配制。

重構

补充2.2 g/L碳酸氢钠。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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