推薦產品
ligand
recombinant protein A (E. coli)
包裝
pack of 5 × 1 mL
製造商/商標名
Cytiva 28-4082-53
儲存條件
(20% Ehtanol)
(20% Ethanol)
參數
42 psi
床尺寸
7 mm × 25 mm
床體積
1 mL
柱內徑
7 mm
基質
highly cross-linked agarose
樣品平均尺寸
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-12
工作範圍
3-10
容量
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
適合性
suitable for bioprocess medium
儲存溫度
2-8°C
一般說明
應用
特點和優勢
- HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
儲存和穩定性
分析報告
其他說明
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
文章
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
條款
Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.
本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
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