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GE17-5318-02

Ni Sepharose 6Fast Flow

Cytiva 17-5318-02, pack of 100 mL

同義詞:

Ni-Sepharose Fast Flow, Nickel Affinity Resin, Nickel Column

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

包裝

pack of 100 mL

製造商/商標名

Cytiva 17-5318-02

基質

6% cross-linked agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning in place

2-14(Ni2+-stripped medium.)

工作範圍

3-12(Ni2+-stripped medium.)

容量

~40 mg binding capacity(histidine-tagged protein)

適合性

suitable for bioprocess medium

一般說明

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity..

Ni Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As A member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

特點和優勢

  • Designed for scaling up purification of histidine-tagged proteins
  • High protein binding capacity and minimal leakage of Ni2? ions
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Environment

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


分析證明 (COA)

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文章

This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

條款

This page shows how to purify histidine-tagged recombinant proteins manually with a syringe using HisTrap FF crude Kit from GE Healthcare.

This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from GE Healthcare.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from GE Healthcare.

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.

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