GE11-0033-99

His GraviTrap^™

Cytiva 11-0033-99

• 同義詞: His GraviTrap Column
• 分類程式碼代碼: 12161501
• NACRES: NA.32

屬性

• 包裝: pkg of 10 ea
• 製造商／商標名: Cytiva 11-0033-99
• 床體積: 1 mL
• 柱內徑: 15 mm
• 基質: 6% cross-linked agarose
• 粒徑: 45-165 μm
• 平均直徑: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium)
• 工作範圍: 3-12(Ni²⁺-stripped medium.)
• 容量: ~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.); ~40 mg binding capacity(histidine-tagged protein)
• 適合性: suitable for bioprocess medium

描述

一般說明

His GraviTrap^™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.

The ready to use column is prepacked with Ni Sepharose^™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.

His GraviTrap^™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.

特點和優勢

• For fast and simple purification of histidine-tagged* proteins.
• Convenient, prepacked gravity-flow column.
• Allows direct purification of both clarified and unclarified cell lysates.
• High binding capacity; approximately 40 mg of pure protein per column.
• Compatible with a wide range of reducing agents, detergents, denaturants and other additives.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

GraviTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全資訊

• 象形圖: GHS09
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids