GE17-3712-06

HisTrap^™ Excel

Cytiva 17-3712-06, pack of 5 × 5 mL

• 同義詞: affinity resins, Ni Sepharose excel column, ion affinity chromatography column, IMAC column
• 分類程式碼代碼: 23151816
• NACRES: NA.56

屬性

• 形狀: prepacked column
• 交聯: cross-linked
• 包裝: pack of 5 × 5 mL
• 製造商／商標名: Cytiva 17-3712-06
• 儲存條件: (20% Ethanol)
• 參數: 5 bar; 5 ml/min - 20 ml/min flow rate
• 技術: liquid chromatography (LC): suitable
• 床尺寸: 16 mm × 25 mm
• 床體積: 5 mL
• 柱內徑: 16 mm
• 基質: highly cross-linked 6% agarose
• 基質活性組: Nickel
• 平均直徑: 90 μm
• cleaning: 2-14
• 工作範圍: 2-13
• 容量: ≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)
• 分離技術: affinity

描述

一般說明

Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.

HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). HisTrap excel 1 mL and 5 mL are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the resin enables fast and convenient purifications. The special type of filter in the top and bottom of the columns makes it possible to load large volumes of cell-free, unclarified samples directly on the columns without causing back pressure problems. This time-saving property helps prevent degradation and loss of sensitive target proteins.

應用

Techniques & Applications: Ready-to-use IMAC columns, prepacked with Ni Sepharose^™ excel medium resistant to chelating and reducing agents

特點和優勢

• Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
• Increase target protein yield and decrease degradation through reduced and simplified sample handling
• HisTrap excel columns allow direct purification of cell-free, unclarified samples
• HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet.
• HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 5 ml/min is recommended.

The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.

法律資訊

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全資訊

• 象形圖: GHS09
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids