GE17-5255-01

HisTrap^™ Fast Flow

Cytiva 17-5255-01, pack of 5 × 5 mL

• 同義詞: HisTrap FF, His-tagged protein purification column, ready-to-use column
• 分類程式碼代碼: 23151816
• NACRES: NA.56

屬性

• 包裝: pack of 5 × 5 mL
• 製造商／商標名: Cytiva 17-5255-01
• 參數: <4 mL/min flow rate; 42 psi
• 床尺寸: 7 mm × 25 mm
• 床體積: 1 mL
• 柱內徑: 7 mm
• 基質: 6% cross-linked agarose
• 粒徑: 45-165 μm
• 平均直徑: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• 工作範圍: 3-12(Ni²⁺-stripped medium.)
• 容量: ~40 mg binding capacity(histidine-tagged protein)
• 適合性: suitable for bioprocess medium

描述

一般說明

HisTrap^™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose^™ 6 Fast Flow matrix make HisTrap^™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap^® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

特點和優勢

• High binding capacity, approx. 40 mg/mL medium.
• Negligible leakage of Ni²⁺.
• Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全資訊

• 象形圖: GHS09
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids