GE17-5268-01

Ni Sepharose^™ High Performance

Cytiva 17-5268-01, pack of 25 mL

• 分類程式碼代碼: 41106500
• NACRES: NA.56

屬性

• 包裝: pack of 25 mL
• 製造商／商標名: Cytiva 17-5268-01
• 基質: highly cross-linked 6% agarose
• 樣品平均尺寸: 34 μm
• cleaning in place: 2-14(Ni²⁺-stripped medium.)
• 工作範圍: 3-12(Ni²⁺-stripped medium.)
• 容量: ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

描述

一般說明

Ni Sepharose^™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni²⁺, which will selectively retain proteins with exposed histidine groups.

The high stability and broad compatibility of Ni Sepharose^™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

特點和優勢

• High-performance purification of histidine-tagged proteins
• Negligible leakage of Ni²⁺ ions.
• High binding capacity, at least 40 mg/mL medium
• Compatible with a wide range of reducing agents, detergents, denaturants and other additives
• 34 μm bead size for improved resolution

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

Sepharose is a trademark of Cytiva

安全資訊

• 象形圖: GHS09
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids