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GE28-9909-44

Sigma-Aldrich

Superdex® 200 Increase 10/300 GL

Cytiva 28-9909-44, column L × I.D. 30 cm × 10 mm, 8.6 μm particle size

同義詞:

Superdex Increase

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

材料

glass column
polyethylene filter

產品線

Superdex®

分子量

10000-600000 Da (globular proteins)

包裝

pkg of 1 ea

製造商/商標名

Cytiva 28-9909-44

參數

<1.8 mL/min flow rate
<1.80 mL/min flow rate
25-500 μL sample volume
3.0 Mpa (580 psi)

床體積

~24 mL

柱長度 × 內徑

30 cm × 10 mm

基質

agarose-dextran composite
polymeric

粒徑

8.6 μm

cleaning

1-14

工作範圍

3-12

分離技術

size exclusion (SEC)

一般說明

Superdex increase 200 是一种用于小规模 (mg) 制备纯化的高分辨率凝胶过滤色谱柱,也可用于表征和分析分子量在 10,000 和 600,000 之间的蛋白质,例如抗体的聚合分析、膜蛋白的粒度均一性分析、 或蛋白质-蛋白质相互作用。预装小的刚性琼脂糖微珠,可实现高流速和 pH 稳定性

特點和優勢

  • Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.
  • Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
  • High flow rates: rigid beads give excellent pressure/flow properties.

適合性

可用于制备和分析目的
用于小规模 (25-500 mL) 制备纯化的凝胶过滤。
长期使用:所有常用水性缓冲液、最高达 8 M 的尿素、离子和非离子洗涤剂、最高达 6M 的盐酸胍

儲存和穩定性

4至30 °C下储存。(20%乙醇)
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

推薦產品

探索最适于 HPLCLC-MS 分析的LiChropur试剂

法律資訊

Superdex is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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分析證明 (COA)

Lot/Batch Number

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文章

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

條款

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

This page shows how to refold proteins using gel filtration and products from GE Healthcare.

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

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