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Key Documents

GE28-9893-35

Sigma-Aldrich

HiLoad® 16/600 Superdex® 200 pg

Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

同義詞:

16/600 Superdex 200, HiLoad Superdex 200

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

材料

bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)

分子量

10000-600000 Da (globular proteins)

包裝

pkg of 1 ea

製造商／商標名

Cytiva 28-9893-35

參數

<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

床體積

120 mL

柱長度 × 內徑

60 cm × 16 mm

基質

agarose dextran composite
 dextran-agarose particle platform
polymeric

基質活性組

dextran-agarose phase

樣品平均尺寸

34 μm

cleaning

1-14

工作範圍

3-12

分離技術

size exclusion (SEC)

一般說明

HiLoad® 16/600 Superdex® 200制备级XK柱设计用于高分辨率制备凝胶过滤色谱。该色谱柱非常适合分离单克隆抗体与二聚体和污染物。

Superdex®制备级(pg)是通过葡聚糖与高度交联琼脂糖的共价结合产生的。这些介质的分离性质由葡聚糖组分决定。陡峭的选择性曲线为分子量范围内的多肽和蛋白质提供了优良的分辨能力：Mr < 10 000 (Superdex® 30 pg)、Mr 3 000至70 000 (Superdex® 75 pg)以及Mr 10 000 600 000 (Superdex® 200 pg)。该培养基结合了高机械强度和高亲水性，允许高流速和极低的非特异性相互作用。

特點和優勢

  • 分辨率高，运行时间短，回收率良好。
  • 高流量下的高容量。
  • 分离可以扩展至生产水平。
  • 预先包装实现了方便和再现性。
  • 1/16”配件方便连接到AKTA设计系统。

適合性

所有常用缓冲液，1 M NaOH、0.1 M HCl、1 M乙酸、8 M尿素、6 M 盐酸胍、1 % SDS、70 %乙醇、30 %异丙醇、30 %乙腈。
用于高分辨率制备凝胶过滤色谱，例如分离mAbs与二聚体和污染物

儲存和穩定性

储存于4至30 °C（20%乙醇）。
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

如需查看本品的分析证书(COA)，请访问www.cytiva.com。

推薦產品

探索最适于 HPLC 或 LC-MS 分析的LiChropur试剂

法律資訊

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Performing an isolation of recombinant protein complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Optimizing Purification of Histidine-Tagged Proteins

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

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Affinity Chromatography in a Purification Strategy (CIPP)

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

Performing a Separation with Superdex

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from GE Healthcare.

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