跳轉至內容
Merck
全部照片(1)

Key Documents

GE28-9893-34

Sigma-Aldrich

HiLoad® 26/600 Superdex® 75 pg

Cytiva 28-9893-34, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size

同義詞:

Size exclusion chromatography

登入查看組織和合約定價


About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

材料

glass column
glass fiber (polypropylene reinforced)

分子量

3000-70000 Da (globular proteins)

包裝

pkg of 1 ea

製造商/商標名

Cytiva 28-9893-34

參數

<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa (42 psi)

床體積

320 mL

柱長度 × 內徑

60 cm × 26 mm

基質

agarose dextran composite
dextran-agarose particle platform
polymeric

基質活性組

dextran-agarose phase
dextran-agarose phase

樣品平均尺寸

34 μm

cleaning

1-14

工作範圍

3-12

分離技術

size exclusion (SEC)

一般說明

HiLoad® 26/600 Superdex® 75 prep grade are prepacked 53 mL XK columns designed for high-resolution preparative gel filtration chromatography separating small proteins and polynucleotides.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

特點和優勢

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

適合性

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

推薦產品

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

法律資訊

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

文章

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

條款

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

This page shows how to refold proteins using gel filtration and products from GE Healthcare.

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務