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Key Documents

S6782

Sigma-Aldrich

Superdex®

Superdex®200 Prep Grade

同義詞:

Size exclusion media, Superdex column

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About This Item

MDL號碼:
分類程式碼代碼:
23151817
NACRES:
NA.56

品質等級

形狀

suspension (20% aqueous ethanol)

技術

protein array: suitable

基質

cross-linked agarose

粒徑

24-44 μm

孔徑

1-100 kDa MW range (dextrans)
10-600 kDa MW range (proteins)

工作pH值範圍

1-14(short term)
3-12(long term)

儲存溫度

2-8°C

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一般說明

将葡聚糖凝胶基质的高选择性与交联琼脂糖的化学和物理稳定性相结合。

應用

Superdex® 可用于蛋白质层析、凝胶过滤层析、凝胶过滤介质、树脂和分离介质。Superdex® 已被用于从食用菌 猴头菇 中纯化和表征一种新型漆酶。Superdex ® 还被用于 紫荆 种子作为潜在人类肝细胞癌抑制剂的纯化和机理研究。
针对尺寸排阻色谱法的高分辨率制备分离进行了优化。列出了球状蛋白和葡聚糖的推荐分馏范围。

法律資訊

Superdex is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

危險分類

Flam. Liq. 3

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

104.0 °F

閃點(°C)

40 °C


分析證明 (COA)

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G Iberer et al.
Journal of chromatography. A, 921(1), 15-24 (2001-07-20)
In size-exclusion chromatography (SEC), proteins and peptides are separated according to their molecular size in solution. SEC is especially useful as an effective fractionation step to separate a vast amount of impurities from the components of interest and/or as final
J Oh et al.
Human molecular genetics, 9(3), 375-385 (2000-02-03)
Hermansky-Pudlak syndrome (HPS) is a rare autosomal recessive disorder in which oculocutaneous albinism, bleeding tendency and a ceroid-lipofuscin lysosomal storage disease result from defects of multiple cytoplasmic organelles: melanosomes, platelet dense granules and lysosomes. The HPS polypeptide, a 700 amino
Marcel Zamocky et al.
The Biochemical journal, 418(2), 443-451 (2008-11-13)
Phytopathogenic fungi such as the rice blast fungus Magnaporthe grisea are unique in having two catalase/peroxidase (KatG) paralogues located either intracellularly (KatG1) or extracellularly (KatG2). The coding genes have recently been shown to derive from a lateral gene transfer from
T Sato et al.
Journal of the American Society of Nephrology : JASN, 10(6), 1242-1252 (1999-06-11)
Mesangial cell (MC) injury is a characteristic feature in the early phase of Thy.1 nephritis. The present study investigates the contribution of complement to MC apoptosis in this experimental model of kidney disease in rats. Thy.1 nephritis was induced by
Anna Lundquist et al.
Biomedical chromatography : BMC, 20(1), 83-87 (2005-06-15)
We studied the effect of charged lipids or detergent on the retention of drugs and an oligonucleotide by immobilized liposome chromatography to characterize solute-membrane interactions. This is a novel approach in analysis of oligonucleotide-liposome interactions. The charged lipids (phosphatidylserine or

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