GE28-4017-45

GSTrap^™ 4B

Cytiva 28-4017-45, pack of 5 × 1 mL

• 分類程式碼代碼: 23151816
• NACRES: NA.56

屬性

• ligand: glutathione
• 形狀: prepacked column
• 交聯: cross-linked
• 包裝: pack of 5 × 1 mL
• 製造商／商標名: Cytiva 28-4017-45
• 儲存條件: (20% Ethanol)
• 參數: <4 mL/min flow rate; 42 psi (H₂O at 25°C)
• 技術: liquid chromatography (LC): suitable
• 床尺寸: 7 mm × 25 mm
• 床體積: 1 mL
• 柱內徑: 7 mm
• 基質: 4% agarose
• 基質活性組: glutathione functional group
• 粒徑: 45-165 μm
• 平均直徑: 90 μm
• cleaning: 4-13
• 工作範圍: 4-13
• 容量: >25 mg binding capacity (horse liver GST/ml medium)
• 分離技術: affinity

描述

一般說明

GSTrap^™ 4B columns are prepacked Glutathione Sepharose^™ 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

應用

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^™ 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

特點和優勢

• High binding capacity.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
• GSTrap^™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA^® design.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

FPLC is a trademark of Cytiva

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

安全資訊

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids