GE28-9523-59

GST Spintrap^™

Cytiva 28-9523-59

• 分類程式碼代碼: 41106500
• NACRES: NA.56

屬性

• ligand: glutathione
• 包裝: pkg of 50 ea
• 製造商／商標名: Cytiva 28-9523-59
• 床體積: 50 μL
• 基質: 4% agarose
• 粒徑: 45-165 μm
• 平均直徑: 90 μm
• cleaning: 4-13
• 工作範圍: 4-13
• 容量: >25 mg binding capacity (horse liver GST/ml medium); ≥500 μg binding capacity(recombinant glutathione S-transferase (rGST))

描述

一般說明

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.

特點和優勢

• Preserves protein antigenicity and function.
• Yields up to 500 μg of pure protein per column.
• Allows direct purification of both clarified and unclarified cell lysates.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (PBS and 0,05% Kathon CG/ICP Biocide)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

SpinTrap is a trademark of Cytiva

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids