推薦產品
ligand
recombinant protein G lacking albumin-binding region
包裝
pkg of 50 × 100 μL
製造商／商標名
Cytiva 28-4083-47
儲存條件
(20% Ethanol)
床體積
100 μL
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning
2-9
工作範圍
3-9
容量
>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
>1 mg binding capacity(IgG/column)
儲存溫度
2-8°C
一般說明
Ab SpinTrap™ are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
應用
Ab SpinTrap™ is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included.
The spin columns are also suitable for use in antibody screening experiments.
Ab SpinTrap™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.
Ab SpinTrap™ contains Protein G Sepharose™ High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.
For optimal performance in antibody purification, use Ab SpinTrap™ together with buffers prepared using Ab Buffer Kit.
The spin columns are also suitable for use in antibody screening experiments.
Ab SpinTrap™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.
Ab SpinTrap™ contains Protein G Sepharose™ High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.
For optimal performance in antibody purification, use Ab SpinTrap™ together with buffers prepared using Ab Buffer Kit.
特點和優勢
- Rapid and convenient small-scale purification of IgG.
- Protein G Sepharose™ High Performance in prepacked spin columns.
- No filtration or centrifugation of different crude samples necessary for antibody purification.
- Classic and crosslink protocols for Protein enrichment (immunoprecipitation).
- Elution conditions formatted for both LC-MS and electrophoresis analysis.
- Elution conditions formatted for both LC-MS and electrophoresis analysis.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
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This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from GE Healthcare.
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