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GE29-0485-81

HiTrap® Protein G HP

Cytiva 29-0485-81, pack of 1 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

包裝

pack of 1 mL

製造商/商標名

Cytiva 29-0485-81

存貨情形

not available in North America

儲存條件

(20% Ehtanol)
(20% Ethanol)

參數

flow rate
42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

基質

highly cross-linked 6% agarose

樣品平均尺寸

34 μm

cleaning

2-9

工作範圍

3-9

容量

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
25 mg binding capacity(human IgG/column)

儲存溫度

2-8°C

一般說明

HiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特點和優勢

High binding capacity of 25 mg IgG/ml medium and fast binding kinetics.
34 μm bead size for high performance, high-resolution purifications.
Binds broad range of different species and subclasses of IgG.
Simple and proven method giving reproducible results.
Designed for use with syringe, peristalic pump, or chromatography system.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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文章

This page describes automated purification of antibodies using ÄKTA chromatography systems.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

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條款

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

This page shows how to prepare samples for purification with affinity chromatography.

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