GE28-9523-60

GST GraviTrap^™

Cytiva 28-9523-60

• 分類程式碼代碼: 41106501
• NACRES: NA.56

屬性

• ligand: glutathione
• 包裝: pkg of 10 ea
• 製造商／商標名: Cytiva 28-9523-60
• 床體積: 2 mL
• 柱內徑: 15 mm
• 基質: 4% agarose
• 粒徑: 45-165 μm
• 平均直徑: 90 μm
• cleaning: 4-13
• 工作範圍: 4-13
• 容量: ≥20 mg binding capacity(recombinant glutathione S-transferase (rGST)); >25 mg binding capacity (horse liver GST/ml medium)

描述

一般說明

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

GST GraviTrap^™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose^™ 4B, sufficient for purification of up to 20 mg of fusion protein.

應用

GST GraviTrap^™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose^™ 4B, sufficient for purification of up to 20 mg of fusion Protein. GST-tagged Proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of prepacked GST GraviTrap^™ columns, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged Proteins.

特點和優勢

• Convenient, prepacked gravity-fLow columns.
• Allows direct purification of both clarified and unclarified cell lysates.
• Yields up to 20 mg of pure Proteins per column.
• Yields up to 20 mg of pure Proteins per column

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

GraviTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全資訊

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids