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D0426

Sigma-Aldrich

DAB with Metal Enhancer

SIGMAFAST™, peroxidase substrate, tablet

同義詞:

3,3′-二氨基联苯胺过氧化物酶底物, DAB过氧化物酶底物

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ DAB，含金属增强剂, tablet

形狀

tablet

品質等級

200

溶解度

water: 1 tablet/5 mL

儲存溫度

−20°C

相關類別

一般說明

使用含金属强化剂的SIGMA FAST™ DAB可制备用于检测过氧化物酶活性的活性底物溶液，无需额外的缓冲液或步骤。常用作免疫组织学的沉淀底物。

應用

含金属强化剂的SIGMAFAST™ DAB 已被用于通过免疫组织化学观察肺组织切片、背根神经节（DRG）神经元和前十字韧带（ACL）细胞中胶原蛋白的免疫组织化学染色。
通过添加CoCl2可以增强DAB反应，并产生更强的耐酒精色斑。

重構

将每个片剂组合溶于5 mL去离子水中，得到含有DAB和金属强化剂的即用型缓冲溶液。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

法律資訊

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Saba Shahin et al.
Toxicological sciences : an official journal of the Society of Toxicology, 161(2), 349-374 (2017-10-27)
Microwave (MW) radiation induced oxidative stress reduces dendritic arborization, spine density and number of hippocampal pyramidal neurons and hence, impair learning and spatial memory through p53-dependent/independent apoptosis of hippocampal neuronal and nonneuronal cells. However, the mechanisms responsible for MW radiation
Somanshu Banerjee et al.
Journal of photochemistry and photobiology. B, Biology, 175, 254-268 (2017-09-20)
Birds time their daily and seasonal activities in synchronization with circadian and annual periodicities in the environment, which is mainly provided by changes in photoperiod/day length conditions. Photoperiod appears to act at the level of eye, pineal and encephalic/deep brain
M Breijo et al.
Journal of economic entomology, 110(3), 1390-1393 (2017-04-08)
The horn fly, Haematobia irritans (L.), is a blood-sucking livestock ectoparasite responsible for substantial livestock losses. In the present work, the potential use of recombinant hematobin (HTB), a horn fly salivary protein, as an antigen for cattle vaccination was investigated.
Platelets and plasma proteins are both required to stimulate collagen gene expression by anterior cruciate ligament cells in three-dimensional culture
Cheng M, et al.
Tissue Engineering: Part A, 16(5), 1479-1489 (2010)
Keith Morris-Schaffer et al.
Particle and fibre toxicology, 16(1), 1-1 (2019-01-08)
Recent epidemiological studies indicate early-life exposure to pollution particulate is associated with adverse neurodevelopmental outcomes. The need is arising to evaluate the risks conferred by individual components and sources of air pollution to provide a framework for the regulation of
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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

條款

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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