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D5637

Sigma-Aldrich

3,3-二氨基联苯胺 四盐酸盐 水合物

≥96%

同義詞:

(1,1′-联苯)-3,3′,4,4′-四胺四盐酸盐二水合物

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About This Item

線性公式:
C12H14N4 · 4HCl · xH2O
CAS號碼:
868272-85-9
EC號碼:
231-018-9
MDL號碼:
MFCD08273058
分類程式碼代碼:
12171500
PubChem物質ID:
57654109
NACRES:
NA.47

品質等級

200

化驗

≥96%

形狀

powder, crystals or chunks

技術

titration: 96-104% (with NaOH)

顏色

white to light brown, and Faint Red and Faint Grey to Light Grey

mp

280 °C

溶解度

H2O: 50 mg/mL

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

−20°C

SMILES 字串

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

InChI 密鑰

DXWSCXIZIHILNP-UHFFFAOYSA-N

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相關類別

一般說明

3,3′-二氨基联苯胺四盐酸盐水合物是一种常用于免疫组织化学和其他染色技术的显色剂，可在生物样品中可视化抗原或抗体的存在。DAB HCl 设计用作免疫组织学染料，以可视化观测细胞中的过氧化物酶活性。可通过分光光度计测量DAB氧化情况。

應用

3,3′-二氨基联苯胺四盐酸盐水合物适用于免疫组织学染色方案、蛋白质印迹和 FISH（荧光原位杂交）。DAB 用于研究早期白内障时的上皮细胞凋亡，以及常染色体显性遗传性多囊肾 (PKD) 的免疫组织化学方案。

生化／生理作用

在过氧化物存在的条件下，DAB用作过氧化物酶反应中的氢供体。氧化后的DAB形成棕色不溶性复合物。

象形圖

Health hazardExclamation mark
GHS08,GHS07

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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