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D7304

Sigma-Aldrich

3,3′-二氨基联苯胺（DAB）液体底物系统 四盐酸盐

同義詞:

Chromogenic substrate, DAB substrate solution

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

形狀

liquid

品質等級

200

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

3,3′-二氨基联苯胺（DAB）是过氧化物酶的发色底物，可作免疫组织学和免疫印迹染色技术的染色剂。最终产品具有耐酒精性，因此DAB液体底物系统可以与多种复染剂和封片剂一起使用。DAB液体底物系统提供了产生快速、便捷的DAB底物溶液所需的所有色原体和缓冲液/过氧化物溶液。

應用

3,3′-二氨基联苯胺（DAB）四盐酸盐液体底物系统已被用作以下检测的底物：
  • 用于鉴定热休克蛋白（Hsp）在肝中表达变化的免疫印迹试验
  • 免疫染色后切片的可视化
  • 小鼠膝关节切片的免疫组织化学
  • 骆驼视前区kisseptin神经元的免疫标记
它与过氧化物酶反应产生的棕色沉淀可用于免疫组织学和免疫印迹染色过程。 不建议用于ELISA（微孔）检测。

注意

可在2-8℃下稳定保存至少一年。

數量

每个 DAB 液体底物系统均提供 250mL 的 DAB 底物溶液。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

僅套裝組件

產品號碼
描述
  • 10X DAB Liquid Chromogen 25 mL
  • ready-to-use buffer/peroxide solution 250 mL

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Carc. 1B - Flam. Liq. 2 - STOT SE 1

標靶器官

Eyes,Central nervous system

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 3

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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存取文件庫

S R Pitkin et al.
Behavioural brain research, 119(2), 167-177 (2001-02-13)
The present study aimed to assess the role of advanced age in the development and manifestation of thiamine deficiency using an animal model of Wernicke-Korsakoff syndrome (WKS). Interactions between pyrithiamine-induced thiamine deficiency (PTD) and age were examined relative to working
K M Mader et al.
Genes, brain, and behavior, 7(7), 740-753 (2008-06-04)
Mucopolysaccharidosis type IIIA (MPS IIIA) is an inherited neurodegenerative lysosomal storage disorder characterised by progressive loss of learned skills, sleep disturbance and behavioural problems. Reduced activity of sulphamidase (SGSH; EC 3.10.1.1) results in intracellular accumulation of heparan sulphate (HS), with
Gamze Tanriover et al.
Clinical breast cancer, 18(5), e1067-e1076 (2018-05-29)
Increased S100A8/A9 expression in Gr1-positive cells has been shown in myeloid-derived suppressor cells and may play a role in the formation of a metastatic milieu. We aimed to determine S100A8/A9 expression alone and with coexpression of Gr1 (a myeloid marker)
J Balthazart et al.
Neuroscience, 153(4), 944-962 (2008-05-02)
Songbirds produce learned vocalizations that are controlled by a specialized network of neural structures, the song control system. Several nuclei in this song control system demonstrate a marked degree of adult seasonal plasticity. Nucleus volume varies seasonally based on changes
Jennifer Walshe et al.
Developmental biology, 264(2), 522-536 (2003-12-04)
Characterisation of human craniofacial syndromes and studies in transgenic mice have demonstrated the requirement for Fgf signalling during morphogenesis of membrane bone of the cranium. Here, we report that Fgf activity is also required for development of the oro-pharyngeal skeleton
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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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