推薦產品
特異性
通过免疫电泳发现本品与抗亲和素(目录号 A5170)反应,与抗链霉亲和素(目录号 S6390)不反应。
應用
ExtrAvidin ®-过氧化物酶适用于以下应用:
- 最低稀释度为 1:4,000 的斑点印迹法
- 免疫组织化学(使用福尔马林固定、石蜡包埋切片,稀释度为 1:100)
- 最低稀释度为 1:2,000 的直接 ELISA
- 免疫沉淀和免疫印迹
- Ra2I 固相结合试验
- Southwestern 印迹法
- 蛋白质印迹法
- 免疫细胞化学
生化/生理作用
抗生物素蛋白 (Avidin) 是一种四聚体或二聚体的生物素结合蛋白,产生于鸟类、爬行动物和两栖动物的输卵管中,并沉积在其蛋清中。由生物素的 4 个高亲和力结合位点组成。ExtrAvidin ® 由蛋清亲和素制备而成。它是亲和纯化亲和素的改良形式,具有亲和素的高比活性和链霉亲和素的低背景染色。是一种由 阿维迪链霉菌 产生的生物素结合蛋白。抗生物素蛋白-过氧化物酶可与生物素以不同的技术联合使用。
外觀
含有0.05% MIT的0.01M磷酸盐缓冲盐水(pH7.4)溶液
準備報告
亲和纯化蛋白
儲存和穩定性
储存在2-8 °C条件下。切勿冷冻。如果长期储存时出现轻微混浊,使用前通过离心澄清溶液。
法律資訊
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
除非我们的产品目录或产品随附的其他公司文件中另有说明,否则我们的产品仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。
訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
客戶也查看了
Scandinavian journal of immunology, 62(4), 361-370 (2005-10-29)
The present study was designed to examine the binding and signalling effects of single base and CpG dinucleotide phosphodiester (Po) oligodeoxynucleotides (ODN) on the human natural killer (NK)-like cell line (YT-INDY). Single base Po ODN composed of 20-mers of guanosine
Journal of leukocyte biology, 91(3), 469-474 (2011-12-15)
M-ficolin specificity for sialylated ligands prompted us to investigate its interactions with the main membrane sialoprotein of human neutrophils, CD43. rM-ficolin bound CD43 and prevented the access of anti-CD43 mAb. Moreover, rM-ficolin reacted exclusively with CD43 on Western blots of
The Journal of clinical investigation, 118(10), 3403-3410 (2008-09-20)
Tregs are important mediators of immune tolerance to self antigens, and it has been suggested that Treg inactivation may cause autoimmune disease. Therefore, immunotherapy approaches that aim to restore or expand autoantigen-specific Treg activity might be beneficial for the treatment
Retrovirology, 14(1), 54-54 (2017-11-29)
Stable insertion of the retroviral DNA genome into host chromatin requires the functional association between the intasome (integrase·viral DNA complex) and the nucleosome. The data from the literature suggest that direct protein-protein contacts between integrase and histones may be involved
Journal of cell science, 108 ( Pt 4), 1629-1637 (1995-04-01)
Integrins alpha 1 beta 1 and alpha 2 beta 1 are major cellular receptors for collagens. The alpha 1 and alpha 2 subunits contain a approximately 200 amino acid inserted domain (I-domain) in their N-terminal region and, because of the
條款
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務