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重要文件

CASP3F

Sigma-Aldrich

胱天蛋白酶3检测试剂盒,荧光法

同義詞:

胱天蛋白酶3(Caspase-3)活性检测试剂盒(荧光法)

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About This Item

EC號碼:
分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)

品質等級

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... CASP3(836)

一般說明

胱天蛋白酶3荧光检测试剂盒的检测原理是通过胱天蛋白酶3(caspase 3)水解乙酰基-Asp-Glu-Val-Asp-7-氨基-4-甲基香豆素(Ac-DEVD-AMC), 释放7-氨基-4-甲基香豆素(AMC),发出荧光。 AMC的激发和发射波长分别为360 nm和460 nm。以AMC标准品制作标准曲线,计算酶解产物AMC浓度(AMC标准品已包含在试剂盒中)。

應用

胱天蛋白酶3荧光检测试剂盒专用于检测细胞提取物或胱天蛋白酶3纯化制品中的胱天蛋白酶3活性。

單位定義

pH 7.4、25 °C下,每单位胱天蛋白酶3每分钟可剪切1.0 μmol Ac-DEVD。

僅套裝組件

產品號碼
描述

  • Water (17-18 megaOhm) 125 mL

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • A1086Ac-DEVD-AMC substrate 2.5 mgSDS

  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS

  • A98917-Amino-4-methylcoumarin standard 1 mgSDS

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


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