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Key Documents

C2409

Sigma-Aldrich

肌酸酶 来源于放线杆菌

lyophilized powder, ≥4 units/mg solid

同義詞:

肌酸脒基水解酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bacterial (Actinobacillus spp.)

品質等級

形狀

lyophilized powder

比活性

≥4 units/mg solid

分子量

~100 kDa

儲存溫度

−20°C

一般說明

肌酸酶是催化肌酸水解的同型二聚体。它由两个单体亚基和两个指定结构域组成;N和C末端结构域。C端折叠在两个结构相似的结构域内,同时具有α螺旋和反平行β折叠。在这两个结构域之间,一个巯基作为活性位点,其活性无金属依赖性。

應用

肌酸酶与肌氨酸氧化酶混合可用于测定不同pH值、温度、酶比和缓冲液浓度下的肌酸水平。也可以通过使用离心分析仪来测定血浆肌酐水平。

生化/生理作用

肌酸酶加速肌酸和水分子转化形成肌氨酸和尿素的反应。它总是以同型二聚体状态起作用,并由氯化胆碱诱导。

物理性質

等电点: 4.6 ± 0.1
米氏常数: 1.9 x 10‾2M(肌酸)
结构: 每摩尔酶2个亚基
抑制剂: Cu++,Hg++,Ag+
最佳pH: 8.0
最佳温度: 40℃
pH稳定性: pH 5.5 − 9.0(25℃,16hr)
热稳定性: 低于50℃(pH 7.5,30分钟)

單位定義

在pH 7.5、37℃条件下,1个单位将每分钟水解1.0 μmol肌酸形成尿素和肌氨酸。

外觀

含有糖和EDTA 作为稳定剂的冻干粉

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Balasundaram Padmanabhan et al.
Acta crystallographica. Section D, Biological crystallography, 58(Pt 8), 1322-1328 (2002-07-24)
The crystal structure of Actinobacillus creatine amidinohydrolase has been solved by molecular replacement. The amino-acid sequence has been derived from the crystal structure. Crystals belong to space group I222, with unit-cell parameters a = 111.26 (3), b = 113.62 (4)
H Crocker et al.
Journal of clinical pathology, 41(5), 576-581 (1988-05-01)
An enzymatic kit method for the determination of plasma creatinine was optimised for use with a centrifugal analyser and its performance characteristics and practicability compared with an end point and a kinetic Jaffé-based method. The enzymatic method exhibited several advantages
J F Bazan et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(7), 2473-2477 (1994-03-29)
Amino acid sequence comparison suggests that the structure of Escherichia coli methionine aminopeptidase (EC 3.4.11.18) and the C-terminal domain of Pseudomonas putida creatinase (EC 3.5.3.3) are related. A detailed comparison of the three-dimensional folds of the two enzymes confirms this
Pinar Esra Erden et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 34(2), 223-239 (2006-03-16)
A new enzyme electrode for the determination of creatine was developed by immobilizing creatinase (CI) and sarcosine oxidase (SO). The enzymes were co-immobilized in a poly(vinylferrocenium) matrix onto the surface of a platinum working electrode. Crosslinking with glutaraldehyte (GA) and
Jason A Berberich et al.
Acta biomaterialia, 1(2), 183-191 (2006-05-17)
The enzyme creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine. Continuous use biosensors are becoming more important in the clinical setting; however, long-use creatinine biosensors have not been commercialized due to

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