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一般說明
辣根过氧化物酶(HRP)是一种重要且被深入研究过的含血红素酶,它是从多年生的草本植物辣根中分离出来的。它使用过氧化氢作为底物,可以催化各种有机和无机化合物进行氧化。HRP参与吲哚-3-乙酸的代谢、生物聚合物的交联和木质化。该酶与吲哚-3-乙酸或其衍生物结合,可作为靶向癌症治疗的潜在疗法。在商业上,HRP可用作临床诊断试剂盒的组成部分,并用于免疫测定。
應用
辣根中的过氧化物酶可用于:
- 用于分离N-连接的聚糖
- 作为D-氨基酸氧化酶(DAAO)的成分缓冲区
- 将过氧化氢(H2O2)分解为水,以证明便携式氧化应激传感器(POSS)的性能
辣根过氧化物酶被广泛用作生物化学中的检测试剂,用于ELISA、免疫印迹和免疫组织化学等多种应用。
單位定義
1个酶活力单位(U)相当于在pH 6.0和25℃下每分钟氧化1 μmol ABTS(货号11557)所需的酶量
其他說明
在苯中使用化学修饰的过氧化物酶;蛋白质的交联;废水中芳香族化合物的酶催化聚合和沉淀
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類(WGK)
WGK 1
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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