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Key Documents

U1500

Sigma-Aldrich

尿素酶 来源于洋刀豆 (刀豆)

Type III, powder, 15,000-50,000 units/g solid

同義詞:

Jack bean urease, Urea amidohydrolase

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物源

Canavalia ensiformis

品質等級

種類

Type III

形狀

powder

比活性

15,000-50,000 units/g solid

分子量

~544620 Da

應用

diagnostic assay manufacturing

儲存溫度

2-8°C

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一般說明

亚基分子量:约 90,770
由六个亚基组成,总分子量:约 544620
脲酶是一种胞质酶,它会催化尿素水解为氨和二氧化碳,首先转化为氨基甲酸酯,然后氨基甲酸酯在水解时进一步分解为氨和碳酸/碳酸盐。 脲酶是含有镍辅因子的金属酶。 脲酶天然存在于植物、真菌和某些细菌中。 詹姆斯·B·萨姆纳(James B.Sumner)于1926年首次从矮刀豆中分离出了脲酶。

生化/生理作用

来自洋刀豆的脲酶通过释放由昆虫肠道中发现的蛋白酶切割的有毒小肽起杀虫剂的作用。

單位定義

一微摩尔单位,在 pH7.0,25℃ 下,每分钟从尿素中释放出 1.0 μmole 的 NH3。它相当于1.0 I.U. 或 0.054 Sumner 单位(在 pH 7.0,20°C,5 分钟内 1.0 mg 氨氮)

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1


分析證明 (COA)

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Fernanda Stanisçuaski et al.
Journal of insect physiology, 55(3), 255-263 (2009-01-06)
Ureases are metalloenzymes that are widespread among plants, fungi and bacteria. Urease isoforms (jack bean urease-JBU and canatoxin) from Canavalia ensiformis seeds are toxic to insects and fungi, suggesting a role in plant defense. The entomotoxic effect is due to
Sun-Il Kim et al.
Microorganisms, 10(1) (2022-01-22)
Ammonia from livestock manure reacts with chemical components discharged from various emission sources to produce airborne particulate matter. This study aimed to investigate a novel effective microbial agent to suppress ammonia gas emitted from manure. Both isolated L12I and 12III
Arlette Yumba-Mpanga et al.
Current medicinal chemistry, 26(1), 216-231 (2017-10-11)
In regard to urogenital tract cancer studies, an estimated 340,650 new cases and 58,360 deaths from genital system cancer and about 141,140 new cases and 29330 deaths from urinary system were projected to occur in the United States in 2012.
Han-Ho Kim et al.
Journal of microbiology and biotechnology, 31(12), 1684-1691 (2021-12-29)
In this study, the physicochemical changes related to fishy smell were determined by storing high hydrostatic pressure (HHP)-treated mackerel (Scomber japonicus) meat in a refrigerator for 20 days. The inhibition of crude urease activity from Vibrio parahaemolyticus using HHP treatment
Nizam Uddin et al.
Fitoterapia, 84, 202-207 (2012-10-30)
Seven constituents were isolated from the stems of Lawsonia alba Lam., following an activity-guided isolation, which include two new constituents, namely lawsorosemarinol (1) and lawsofructose (2), one known compound 2-(β-d-glucopyranosyloxy)-1, 4-naphthoquinone (3) and four compounds, 4-hydroxy coumarine (4), 3-(4-hyroxyphenyl)-triacontyl-(Z)-propenoate (5)

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