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重要文件

U0251

Sigma-Aldrich

尿素酶 来源于洋刀豆 (刀豆)

Type C-3, powder, ≥600,000 units/g solid

同義詞:

Jack bean urease, Urea amidohydrolase

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物源

Canavalia ensiformis

品質等級

種類

Type C-3

形狀

powder

比活性

≥600,000 units/g solid

分子量

~544620 Da

純化經由

crystallization

儲存溫度

−20°C

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一般說明

亚基分子量:约 90,770
由六个亚基组成,总分子量:约 544620
尿素酶是一种依赖镍的金属酶,存在于各种植物、细菌、真菌和藻类中。它含有两个不同的亚基——UreA(26.5 kDa)和UreB(60.3–61.6 kDa)。

生化/生理作用

在 Hela 细胞中,脲酶可诱导空泡化,这种作用取决于脲酶将尿素转化为氨的能力。
尿素酶有助于植物中氮的生物利用。它与防御机制有关,具有杀虫活性。此外,尿素酶可通过抑制丝状真菌和酵母的生长和影响细胞膜完整性表现出抗真菌活性。

單位定義

一微摩尔单位,在 pH7.0,25℃ 下,每分钟从尿素中释放出 1.0 μmole 的 NH3。它相当于1.0 I.U. 或 0.054 Sumner 单位(在 pH 7.0,20°C,5 分钟内 1.0 mg 氨氮)

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1


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T L Cover et al.
Infection and immunity, 59(4), 1264-1270 (1991-04-01)
Concentrated broth culture supernatants from 50 to 60% of Helicobacter pylori strains induce eukaryotic cell vacuolation in vitro. A quantitative assay for cell vacuolation was developed on the basis of the rapid uptake of visibly vacuolated HeLa cells was significantly
Barbara Krajewska et al.
Plant physiology and biochemistry : PPB, 43(7), 651-658 (2005-07-19)
A pH-variation study of jack bean (Canavalia ensiformis) urease steady-state kinetic parameters and of the inhibition constant of boric acid, a urease competitive inhibitor, was performed using both noninhibitory organic (MES, HEPES and CHES) and inhibitory inorganic (phosphate) buffers, in
Yu Hang Fong et al.
PLoS biology, 11(10), e1001678-e1001678 (2013-10-12)
Urease is a metalloenzyme essential for the survival of Helicobacter pylori in acidic gastric environment. Maturation of urease involves carbamylation of Lys219 and insertion of two nickel ions at its active site. This process requires GTP hydrolysis and the formation
Angela R Piovesan et al.
Archives of biochemistry and biophysics, 547, 6-17 (2014-03-04)
Ureases catalyze the hydrolysis of urea into NH3 and CO2. They are synthesized by plants, fungi and bacteria but not by animals. Ureases display biological activities unrelated to their enzymatic activity, i.e., platelet and neutrophil activation, fungus inhibition and insecticidal
Anne H S Martinelli et al.
Biochimica et biophysica acta, 1840(3), 935-944 (2013-11-19)
Ureases are metalloenzymes involved in defense mechanisms in plants. The insecticidal activity of Canavalia ensiformis (jack bean) ureases relies partially on an internal 10kDa peptide generated by enzymatic hydrolysis of the protein within susceptible insects. A recombinant version of this

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