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重要文件

U7752

Sigma-Aldrich

尿素酶 来源于洋刀豆 (刀豆)

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物源

Canavalia ensiformis

品質等級

形狀

solid

分子量

hexamer 545 kDa
trimer ~272 kDa

包含

~20% dithiothreitol

適合性

suitable for marker for electrophoresis (non-denaturing PAGE)

儲存溫度

−20°C

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一般說明

亚基分子量:约 90,770
由六个亚基组成,总分子量:约 544620
尿素酶(脲酶)是一种多亚基的镍依赖型金属酶。大量存在于真菌、酵母、植物和细菌中。属于酰胺水解酶和磷酸三酯酶超家族。尿素酶也存在于豆科植物种子中。

應用

洋刀豆尿素酶已用于:
  • 作为电泳的分子量标准品组分
  • 作为体积排阻色谱的标准品组分
  • 作为天然聚丙烯酰胺凝胶电泳(PAGE)的参比物质

生化/生理作用

尿素酶是氮循环的必需酶。它催化尿素分解成为氨和甲酸。尿素酶为各种生物提供生长所需氮源。在种子发芽和种子化学防御中同样发挥重要作用。

其他說明

包含约1 mg蛋白质

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1


分析證明 (COA)

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Muhammad Ajmal Rana et al.
PloS one, 16(10), e0258568-e0258568 (2021-10-15)
Urea is the most popular and widely used nitrogenous fertilizer. High soil urease activity rapidly hydrolyses applied urea to ammonia which contributes to soil nitrogen (N) losses and reduces N use efficiency of crop plants. The ammonia losses can be
Anuradha Balasubramanian et al.
Journal of molecular biology, 400(3), 274-283 (2010-05-18)
Urease, a nickel-dependent metalloenzyme, is synthesized by plants, some bacteria, and fungi. It catalyzes the hydrolysis of urea into ammonia and carbon dioxide. Although the amino acid sequences of plant and bacterial ureases are closely related, some biological activities differ
Prevention of infected urinary stones by urease inhibition.
D P Griffith et al.
Investigative urology, 11(3), 228-233 (1973-11-01)
Paweł Kafarski et al.
Journal of advanced research, 13, 101-112 (2018-08-11)
Urease is a nickel-dependent metalloenzyme found in plants, some bacteria, and fungi. Bacterial enzyme is of special importance since it has been demonstrated as a potent virulence factor for some species. Especially it is central to Helicobacter pylori metabolism and
Suchithra Menon et al.
Cell, 156(4), 771-785 (2014-02-18)
mTORC1 promotes cell growth in response to nutrients and growth factors. Insulin activates mTORC1 through the PI3K-Akt pathway, which inhibits the TSC1-TSC2-TBC1D7 complex (the TSC complex) to turn on Rheb, an essential activator of mTORC1. However, the mechanistic basis of how

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