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B3679

Sigma-Aldrich

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

同義詞:

5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

產品名稱

用于膜的BCIP®/NBT-紫色液体底物系统, ready to use alkaline phosphatase substrate

品質等級

100

形狀

liquid

儲存溫度

2-8°C

相關類別

應用

BCIP®/NBT-紫色液体膜底物系统已被用作碱性磷酸酶的底物
  • 用于通过免疫印迹法检测二抗
  • 用于ELISPOT测定分析
  • 用于斑点印迹分析
  • 用于免疫印迹分析，以可视化硝酸纤维素膜上的蛋白条带

生化／生理作用

5-溴-4-氯-3-吲哚基磷酸酯 (BCIP)/硝基蓝四唑（NBT）/硝基蓝四唑鎓(NBT)是用于检测碱性磷酸酶(AP)的显色底物系统。AP水解BCIP形成中间体无色靛蓝化合物，并进一步被NBT氧化形成5,5′-二溴-4,4′-二氯靛蓝(BCI)。其中，NBT被还原为不溶的紫色双甲双胍(DF)，形成NBT-DF。NBT-DF和BCI的混合物形成深紫色沉淀。AP可水解多种BCIP分子，因此成为了一种高灵敏度方法。

注意

在2-8 °C稳定至少一年。

外觀

即用型。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H317

危險分類

Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
MKCH2415
MKCH2414
MKCH2413
MKCF9800
MKCF9801

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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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