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Merck
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重要文件

A8025

Sigma-Aldrich

抗 兔 IgG(全分子)-碱性磷酸酶 山羊抗

affinity isolated antibody, buffered aqueous glycerol solution

同義詞:

Goat Anti-Rabbit IgG (whole molecule)–AP

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

品質等級

共軛

alkaline phosphatase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous glycerol solution

物種活性

rabbit

技術

direct ELISA: 1:7,000

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

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一般說明

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody binds to all rabbit Igs.
Immunoglobulin G (IgG) is part of the immunoglobulin family and is widely expressed in the serum. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric structure of IgG consists of two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity (Fab fragments). The third fragment known as fragment crystallizable (Fc).

免疫原

purified rabbit IgG

應用

通过 ELISA 法分析 PADI4 对抗凝血酶的瓜氨酸化作用,使用碱性磷酸酶标记的羊抗兔 IgG 作为在0.05M碳酸盐/碳酸氢盐缓冲液(Ph 9.6)中以1:5000稀释的二次稀释剂。
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Goat Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for ELISA assays at a dilution of 1:5,000.
Protein expression in maize endosperm amyloplast preparations or whole cell lysates were analyzed by alkaline phosphatase-conjugated goat anti-rabbit IgG as the secondary antibody.

外觀

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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Immunobiology (2001)
Graham H Cowan et al.
Frontiers in plant science, 3, 290-290 (2012-12-28)
The potato mop-top virus (PMTV) triple gene block 2 (TGB2) movement proteins fused to monomeric red fluorescent protein (mRFP-TGB2) was expressed under the control of the PMTV subgenomic promoter from a PMTV vector. The subcellular localizations and interactions of mRFP-TGB2
Jens Madsen et al.
PloS one, 8(5), e64441-e64441 (2013-05-22)
The protein deleted in malignant brain tumors (DMBT1) and the trefoil factor (TFF) proteins have all been proposed to have roles in epithelial cell growth and cell differentiation and shown to be up regulated in inflammatory bowel diseases. A panel
M C de Beer et al.
The Biochemical journal, 283 ( Pt 3), 673-678 (1992-05-01)
Four serum amyloid A protein (SAA) genes and two gene products, apo-SAA1 and apo-SAA2 were identified in BALB/c mice (type A). SJL/J mice (type B) are thought to be defective in apo-SAA2 expression. A unique variant of mouse apo-SAA was
J Keener et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(25), 13458-13462 (1998-02-12)
RNA polymerase I (Pol I) transcription in the yeast Saccharomyces cerevisiae is greatly stimulated in vivo and in vitro by the multiprotein complex, upstream activation factor (UAF). UAF binds tightly to the upstream element of the rDNA promoter, such that

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