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B3804

Sigma-Aldrich

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

同義詞:

5-溴-4-氯-3-吲哚磷酸酯/硝基蓝四唑液体底物系统

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

product name

BCIP®/NBT-蓝色液体膜底物系统, alkaline phosphatase substrate

品質等級

100

形狀

liquid

儲存溫度

2-8°C

相關類別

一般說明

硝基蓝四唑（NBT）与碱性磷酸酶底物5溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于免疫印迹和免疫组织学染色程序。这些底物系统能够产生不溶的NBT二甲双胍终产物，呈蓝色至紫色并且可以在视觉上观察到。BCIP®/NBT蓝液底物系统是一种专门配制的稳定液体酶底物，针对印迹应用进行了优化，方便省时，一致性高。

應用

BCIP®/NBT蓝液底物系统已用于碱性磷酸酶(ALP)活性的比色检测，如蛋白质印迹分析。

注意

在2-8°C下可稳定至少一年。该基底具有光敏性。

其他說明

不建议微孔(如ELISA)或免疫组化应用。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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