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一般說明
碱性磷酸酶是一种金属酶，属于外切核苷酸酶家族。存在于胆管、骨、肠、胎盘和肿瘤中。
應用
在与碱性磷酸酶反应之前，BCIP®试剂为无色至淡蓝色，硝基BT试剂为黄色。在膜型测定中，两组分混合物与碱性磷酸酶形成蓝紫色产物。不建议将该底物用于ELISA分析。
碱性磷酸酶蓝膜底物溶液已用于：
- 分析直接碱性磷酸酶（AP）活性
- 用比色系统进行碱性磷酸酶测定
- 分析诱导多能干细胞（iPSC）系LND554SV.3的直接碱性磷酸酶（AP）活性
外觀
2组分试剂盒足以制备400 mL工作底物。
作为含溴氯吲哚磷酸盐（BCIP®）和硝基蓝四唑（NBT/噻唑蓝/硝基BT）的双组分缓冲碱性磷酸酶底物提供
法律資訊
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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文章
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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