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Key Documents

AP0100

Sigma-Aldrich

碱性磷酸酶二乙醇胺活性检测试剂盒

sufficient for 100 tests

同義詞:

ALP Activity Kit

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.26

用途

sufficient for 100 tests

品質等級

儲存溫度

2-8°C

一般說明

碱性磷酸酶二乙醇胺检测试剂盒提供了一种检测碱性磷酸酶活性的即用型试剂。这种简单的检测分析碱性磷酸酶活性的方法使用了p-磷酸对硝基苯酯(pNPP)作为底物。

應用

碱性磷酸酶活性二乙醇胺法检测试剂盒已被用于:
  • 检测血清和成骨细胞中碱性磷酸酶(ALP)的水平
  • 监测小鼠成肌细胞C2C12细胞在成骨细胞分化过程中的ALP水平
  • 测定肺癌骨转移模型中的ALP活性
  • 测定结肠组织匀浆中的ALP水平

適合性

适用于多种样品中碱性磷酸酶活性的测定。

原則

碱性磷酸酶将pNPP水解为对硝基苯酚和无机磷酸盐。在37°C下孵育碱性磷酸酶样品和底物期间,通过监测405 nm处吸光度的增加来追踪反应。

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • Alkaline Phosphatase Diethanolamine Reaction Buffer

  • P4744Phosphatase substrate, powderSDS

  • P6774Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous solution, ≥2,000 DEA units/mg proteinSDS

象形圖

Health hazardCorrosion

訊號詞

Danger

危險分類

Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

標靶器官

Kidney,Liver,Blood

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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