Skip to Content
Merck

Improved profiling of estrogen metabolites by orbitrap LC/MS.

Steroids (2014-12-30)
Xingnan Li, Adrian A Franke
ABSTRACT

Estrogen metabolites are important biomarkers to evaluate cancer risks and metabolic diseases. Due to their low physiological levels, a sensitive and accurate method is required, especially for the quantitation of unconjugated forms of endogenous steroids and their metabolites in humans. Here, we evaluated various derivatives of estrogens for improved analysis by orbitrap LC/MS in human serum samples. A new chemical derivatization reagent was applied modifying phenolic steroids to form 1-methylimidazole-2-sulfonyl adducts. The method significantly improves the sensitivity 2-100 fold by full scan MS and targeted selected ion monitoring MS over other derivatization methods including, dansyl, picolinoyl, and pyridine-3-sulfonyl products.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Testosterone, ≥98%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Progesterone, meets USP testing specifications
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dansyl chloride, BioReagent, suitable for amino acid labeling, powder and chunks, ≥99% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Progesterone, γ-irradiated, BioXtra, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Progesterone, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Progesterone, powder, BioReagent, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
2-Picolinic acid, ReagentPlus®, 99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dansyl chloride, suitable for fluorescence, BioReagent, ≥99.0% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Testosterone, purum, ≥99.0% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
2-Methyl-6-nitrobenzoic anhydride, 97%
SKU
Pack Size
Availability
Price
Quantity
Supelco
Testosterone solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Pyridine-3-sulfonyl chloride, 95%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
2-Methoxyestradiol, powder
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
4-Hydroxyestradiol
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
4-Hydroxyestrone, ≥90% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetonitrile, ≥99.5%, ACS reagent
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Pyridine, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Formic acid, ≥95%, FCC, FG
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetonitrile, electronic grade, 99.999% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Pyridine, anhydrous, 99.8%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
4-(Dimethylamino)pyridine, ReagentPlus®, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
1-Methylimidazole-2-sulfonyl chloride, 95%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Ultrapure Acetonitrile
SKU
Pack Size
Availability
Price
Quantity