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Merck

PP0201

Sigma-Aldrich

Glycoprofile II, Enzymatic In-Solution N-Deglycosylation Kit

sufficient for 20 samples

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.26

儲存溫度

2-8°C

一般說明

含有足够进行至少20次反应所需的试剂,对于典型的糖蛋白,可处理样品范围为1~2 mg。

應用

GlycoProfile II Enzymatic In-Solution N-去糖基化试剂盒用于研究裂褶菌中N-乙酰-D-半乳糖胺(GalNAc)特异性凝集素的糖基化作用。
GlycoProfile II经过优化,可提供一种方便且可重复的从糖蛋白中去除N-连接聚糖的方法,与后续的MALDI-TOF质谱分析兼容,而不受任何反应成分的干扰。
Glycoprofile II, Enzymatic In-Solution N-去糖基化试剂盒已用于裂褶菌凝集素、拟南芥甘露聚糖酶、人脐静脉内皮细胞(HUVEC)蛋白裂解物和有机溶质转运蛋白Ostβ的去糖基化。

法律資訊

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明

  • 10× Reaction Buffer 1 mL/vial

试剂盒组分也可单独购买

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訊號詞

Danger

危險分類

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

標靶器官

Liver,Heart

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials


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Christen Y L Yuen et al.
Plant & cell physiology, 58(6), 1103-1117 (2017-04-27)
Members of the protein disulfide isomerase (PDI)-C subfamily are chimeric proteins containing the thioredoxin (Trx) domain of PDIs, and the conserved N- and C-terminal Pfam domains of Erv41p/Erv46p-type cargo receptors. They are unique to plants and chromalveolates. The Arabidopsis genome
Enzymatic characterization of a glycoside hydrolase family 5 subfamily 7 (GH5\_7) mannanase from Arabidopsis thaliana
Wang Y, et al.
Planta, 239(3), 653-665 (2014)
beta-Subunit of the Ostalpha-Ostbeta organic solute transporter is required not only for heterodimerization and trafficking but also for function
Christian WV, et al.
Test, 287(25), 21233-21243 (2012)
Podjana Chumkhunthod et al.
Biochimica et biophysica acta, 1760(3), 326-332 (2006-03-02)
An N-acetyl-D-galactosamine (GalNAc)-specific lectin was purified from the edible mushroom, Schizophyllum commune, using affinity chromatography on a porcine stomach mucin (PSM)-Sepharose 4B column. Under reducing and non-reducing conditions, SDS-polyacrylamide gel electrophoresis gave a major band of 31.5 kDa. The Schizophyllum
Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown
Haarmann A, et al.
PLoS ONE, 5(10), e13568-e13568 (2010)

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