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重組細胞
expressed in E. coli
品質等級
共軛
(N-linked)
等級
Proteomics Grade
形狀
ready-to-use solution
運輸包裝
wet ice
儲存溫度
2-8°C
一般說明
肽-N-糖苷酶F（PNGase F）属于酶家族，主要用于N-连接聚糖的去糖基化。
應用
PNGase Fast可用于固定以进行去糖基化。它也可用于固定在基于甲基丙烯酸酯的整体载体上，以从糖蛋白释放N-连接的碳水化合物部分。
生化／生理作用
肽-N-糖苷酶F（PNGase F）从糖蛋白上裂解天冬酰胺连接的高甘露糖，杂合和复杂寡糖。它也可以使天冬酰胺脱氨基为天冬氨酸。PNGase Fast能够对抗体和免疫球蛋白融合蛋白，以及其他糖蛋白进行完整和快速的去糖基化，以准备用于下游层析或质谱分析。PNGase Fast创建了优化的工作流程，在不损害灵敏度或重现性的情况下减少了处理时间。
儲存類別代碼
10 - Combustible liquids
其他客户在看
David A Fischler et al.
Journal of biomolecular techniques : JBT, 30(4), 58-63 (2019-10-11)
There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization. One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F). A less expensive and quicker alternative has been reported
Kathleen L Plona et al.
JIMD reports, 60(1), 56-66 (2021-07-15)
Due to advances in sequencing technologies, identification of genetic variants is rapid. However, the functional consequences of most genomic variants remain unknown. Consequently, variants of uncertain significance (VUS) that appear in clinical DNA diagnostic reports lack sufficient data for interpretation.
商品
The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.
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