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Merck

P7367

Sigma-Aldrich

糖苷酶F 来源于脑膜脓毒性伊丽莎白菌

BioReagent, ≥95% (SDS-PAGE), for proteomics

别名:

N-糖苷酶F, 糖苷酶F 来源于脑膜炎脓毒性黄杆菌, 糖苷酶F 来源于脑膜脓毒性金黄杆菌, 肽N-糖苷酶

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.32

生物源

bacterial (Elizabethkingia meningoseptica)

品質等級

共軛

(N-linked)

等級

for proteomics

產品線

BioReagent

化驗

≥95% (SDS-PAGE)

形狀

powder

儲存期限

≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml)
≥1 yr at 2‑8 °C
Solution is stable for at least 3 freeze-thaw cycles

分子量

~36 kDa

濃度

≥300 units/mL
≥50 units/mL

最適pH

~8.6

運輸包裝

wet ice

儲存溫度

2-8°C

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相关类别

一般說明

蛋白质组学级糖苷酶F经过从稀磷酸钾缓冲液中充分纯化和冻干,以生产出稳定的产品。 本品不含甘油和其他稳定剂,并且缓冲盐含量非常低。 这种高度纯化的材料非常适用于凝胶、溶液或印迹膜中糖蛋白或糖肽的N-连接去糖基化。

應用

来源于脑膜脓毒性黄杆菌(Elizabethkingia meningoseptica) 的PNGase F已被用于:
  • 寨卡20病毒E蛋白的去N-糖基化
  • 采用表达 CAR 的 COS 细胞评估柯萨奇病毒和腺病毒受体的糖基化
  • 验证 MHC 1 类多肽相关序列 A(MICA)的 N连接糖基化

适用于蛋白质组学和糖生物学;与MALDI-TOF MS分析相容
用于使蛋白质去糖基化。

生化/生理作用

从糖蛋白切割整个聚糖,前提是糖基化天冬酰胺部分在其氨基和羧基末端被多肽链取代。

單位定義

一个酶活性单位是指在37℃、pH 7.5条件下通过SDS-PAGE监测,在1分钟内催化1nM变性核糖核酸酶B释放N-连接寡糖所需的酶量。 糖苷酶F活性的一个Sigma单位相当于1 IUB毫单位。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Katherine J D Ashbourne Excoffon et al.
Journal of virology, 81(11), 5573-5578 (2007-03-23)
The coxsackievirus and adenovirus receptor (CAR) is both a viral receptor and homophilic adhesion protein. The extracellular portion of CAR consists of two immunoglobulin (Ig)-like domains, each with a consensus sequence for N-glycosylation. We used chemical, genetic, and biochemical studies
Lars Andresen et al.
Journal of immunology (Baltimore, Md. : 1950), 188(4), 1847-1855 (2012-01-10)
NKG2D ligand surface expression is important for immune recognition of stressed and neotransformed cells. In this study, we show that surface expression of MICA/B and other NKG2D ligands is dependent on N-linked glycosylation. The inhibitor of glycolysis and N-linked glycosylation
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Royalactin is a glycoprotein essential for the development of long-lived queen honeybees. Only larvae fed with royal jelly, containing royalactin, develop into queens. Royalactin plays a central role in this process by switching on the epidermal growth factor (EGF) receptor
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商品

N-Linked Glycan Strategies; Sigma-Aldrich.com

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